Featuring PermaRed/AP-Auto
Traditional formulations for red alkaline phosphatase chromogens are unstable upon mixing. These working solutions must be used fresh. After 15 minutes has elapsed, the color development drastically decreases. This can be problematic for use in automated slide stainers without on-board reagent mixing stations. The user must return to the instrument to add freshly made working solution. Often, the 15 minute reaction window will be exceeded before the substrate/chromogen is applied to the slide due to program delays for washes and timing pauses. This leads to poor and inconsistent staining.

PermaRed/AP-Auto has been formulated for on-slide mixing. The instrument should be programmed to apply the substrate-buffer to the slide followed by application of the chromogen reagent. The substrate—buffer and chromogen components are stable in their separate formats. They can be loaded into the reagent rack at the beginning of the staining run with the other reagents, for walk away convenience.

PermaRed/AP-Auto produces a red color, ranging from pink to brilliant dark red, depending on the signal intensity. It is insoluble in organic solvents; therefore sections can be dehydrated in alcohol, cleared in xylene (or xylene substitute) and permanently mounted.
Catalog No.: K 049-AUTO Ready to Use 30 ml, K049-AUTO-110 Ready to Use 110 ml

Stable DAB/Plus
DAB, a widely used chromogen for immunoperoxidase staining, is well accepted among pathologists because of its high sensitivity and cleaner background than aminoethylcarbazole (AEC). Specimens stained in DAB can be dehydrated, cleared and mounted for permanent record keeping. Stable DAB/Plus is more sensitive and stable than traditional working DAB solutions, which reduces hazardous waste generation.
Catalog No.: K 047 Ready to Use 5 ml, K 047-200 Ready to Use 200 ml

PermaBlue/AP (Alcohol and Xylene Compatible)
PermaBlue/AP is a substrate-chromogen system designed to be used for either IHC or ISH when utilizing alkaline phosphatase. PermaBlue/AP produces a vibrant blue color. PermaBlue/AP is insoluble in alcohol and xylene substitutes (both aliphatic hydrocarbon and citrus based); therefore sections can be dehydrated in alcohol, cleared in xylene substitute and permanently mounted.
Catalog No.: K 051 Ready to Use 30 ml, K 051-110 Ready to Use 110 ml

Other Chromogens by Diagnostic BioSystems Include:

PermaRed/AP Catalog No.: K 049 Ready to Use 30 ml, K 049-110 Ready to Use 110 ml

PermaGreen/AP Catalog No.: K 052 Ready to Use 30 ml, K 052-110 Ready to Use 110 ml

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